Figure 3
From: Repetitive part of the banana (Musa acuminata) genome investigated by low-depth 454 sequencing
Genomic distribution of different types of DNA repeats. Mitotic metaphase spreads of M. acuminata cv. 'Calcutta 4' (2n = 22) after FISH with probes for various repeats. The chromosomes were counterstained with DAPI (blue). Bar = 5 μm. (A) Tandem repeat CL18 (green signal) formed a cluster on one pair of chromosomes (long arrows). (B) Tandem repeat CL33 (red signal) localized on two pairs of chromosomes (long arrows). (C) Simultaneous hybridization of probes for CL18 (green signal) and CL33 (red signal, long arrows) revealed co-localization of both satellites on one pair of chromosomes (short arrows). (D) Two metaphase plates after FISH with a probe for CL1SCL2Contig1080 - banana retrotransposon belonging to SIRE/Maximus lineage (green). Uneven genomic distribution with clusters dispersed on all chromosomes is obvious. (E) Similar genomic distribution was found for banana retroelement related to the Angela lineage (CL2Contig49). (F) Banana retrotransposon belonging to Tnt1 lineage (CL10Contig16, red color) gave weak signals preferentially localized in distal parts of chromosomes (long arrows). (G) The most abundant type of Ty3/gypsy-like element of the Reina lineage (CL1SCL5Contig891) localized preferentially to centromeric or peri-centromeric regions of all chromosomes (green signals). (H) Also the Ty3/gypsy-like element related to Tekay evolutionary lineage (CL4Contig82) clustered in centromeric or peri-centromeric regions of all chromosomes. (I) A probe derived from LINE element (CL1SCL8Contig452) localized in the centromeric regions of all chromosomes (green signals).