Figure 2
From: Co-option of EDM2 to distinct regulatory modules in Arabidopsis thalianadevelopment
EDM2 and WNK8 are required for leaf pavement cell development. A: Scanning electron microscopy with adaxial surfaces of the 6th rosette leaves of the indicated Arabidopsis genotypes. 25 day-old plants grown under long-day conditions were used. The red scale bar represents 200 μm. pXVE:HA-EDM2-a plants were not treated with estradiol. B: Circularity [c = 4pi × (area/perimeter2)] as a numerical expression of cell shape. C: Average circularity values of 40 - 80 cells per genotype determined from the photos shown in (A) using ImageJ http://rsbweb.nih.gov/ij/. Significant deviations of values from the tested mutants to their respective wild type controls (Col-5 for edm2-1, Col-0 for all others) based on t-tests (p < 0.05) are denoted by asterisks.