Transfer RNA modifications and genes for modifying enzymes in Arabidopsis thaliana

BMC Plant Biology

Table 4 Quantification of modified nucleosides in T-DNA mutants.

T-DNA line (NASC line)	Gene	m¹G/Ψ	m⁷G/Ψ	ncm⁵U/Ψ	m²G/Ψ
wt		0.344	0.338	0.014	0.273
N653345	At5g47680 (AtTRM10)	0.185 (53%)
N665836	At5g47680 (AtTRM10)	0.208 (60%)
N658418	At1g03110 (AtTRM82)		< 0.001 (< 0.2%)
N661341	At5g13680 (AtELP1)			< 0.0002 (< 0.01%)
N658947	At1g13870 (AtKTI12)			< 0.0003 (< 0.02%)
N622158	At3g26410 (AtTRM11)				0.020 (7.3%)

Amount of certain modified nucleosides were shown using relative quantification to internal standard (Ψ), percentage of wild type level was shown in bracket.

ISSN: 1471-2229