Skip to main content
Figure 5 | BMC Plant Biology

Figure 5

From: Characterization of bacterial-type phosphoenolpyruvate carboxylase expressed in male gametophyte of higher plants

Figure 5

Protein and gene expression of AtBTPC. (A) Schematic view of the gene structure of genomic Atppc4 (At1g68750) used for expression analysis. Black boxes represent exons. The Venus gene was inserted at the Bst1107I site in the last exon. (B) Arabidopsis flower buds. The numbers indicate the flower bud length (mm). Scale bar = 0.5 mm. (C, D) DAPI staining of pollen in 1.2 mm flower bud (1.2FB; C) and in 1.5 mm flower bud (1.5FB; D). VN, vegetative cell nucleus; GCN, generative cell nucleus; SCN, sperm cell nucleus. Scale bar = 10 μm. (E) CLSM image of Venus fluorescence in pollen at stage 1.5FB. The blank regions correspond to VN and SC. Scale bar = 10 μm. (F-I) CLSM images of Venus fluorescence in pollen within an anther at stages 1.2FB-2.2FB. Hemizygous T3 plants were used for the analysis. Each number in the bottom right corner represents the relative fluorescence intensity. Note that the relative fluorescence intensity of the pollen at 1.2FB is given as 1.0, because there was no fluorescent signal in the transgenic pollen at this stage. (J) Immunoblot analysis with anti-GFP antibody of flower buds from homozygous T3 plants. Flower buds were analyzed at stages before 1.2FB (≤ 1.2FB) at 1.5FB-1.8FB, and after 2.2FB (≥ 2.2FB). The arrowhead indicates a band of AtPPC4-Venus protein. The lower bands with star marks in all lanes are nonspecific bands. (K) RT-PCR of Atppc4 in flower buds at different stages from wild-type plants. The stages of the flower buds are the same as in (J). Arabidopsis elongation factor 1α(AtEF1α, TAIR: At1g07920) was used as the standard.

Back to article page