QTLs and candidate genes for desiccation and abscisic acid content in maize kernels

BMC Plant Biology

Table 4 Maize NCED and ZEP gene mapping and colocation with QTLs for desiccation and/or ABA content

Gene Name	EST	IGR ID^a HTGS ID^b yrGATE ID^c	Map	Bin	MM coord.^d	Proj. coord.^e	Flanking markers	QTL colocation
NCED1	[GenBank:ZMU95953] E746	Vp14 [GenBank:AC230016.2]	LHFR_Gnp2004	1.08- 1.09	504	466	bnlg1643- phi055	DW_80_3 FW_80_2 Water_80_1
NCED2	E746	AZM4_115740 [GenBank:AC217286.3]	LHFR_Gnp2004	1.05- 1.06	328	306	umc 1906- umc 67	Water_60_1 %DW_60_2
NCED3	No	AZM4_115695 [GenBank:AC199036.2]	LHFR_Gnp2004	3.05- 3.06	193	207	umc1501- dupssr23	No
NCED4	No	AZM4_114127 [GenBank:AC212820.3]	LHFR_Gnp2004	3.00	-108.7	-125	Before umc1746	No
NCED5	[GenBank:QCD5h12]	AZM4_50254 [GenBank:AC194862.3]	LHFR_Gnp2004	5.06- 5.07	364	298	phi087- umc1537	%DW_80_3 ABA_embryo_40_3 DW_60_4
NCED6	No	AZM4_50252 [GenBank:AC190588.3]	REFMAP050110	4.06	177	177	bnlg 1621- umc 66a	%DW_30_2
ZEP1	QAG5c10 [GenBank:AI977858]	AZM5_13314 [GenBank:AC194845.3] yrGATE_Zm2 gZEP1	REFMAP050110	2.04	221	221	bnlg1018- bnlg166	ABA_embryo_30_1 ABA_embryo_40_2 ABA_endosperm_40_1 Slope_1
ZEP2	QAG5c10	AZM5_13312 [GenBank:AC206194.3] yrGATE_Zm10 gZEP2	REFMAP050110	10.04- 10.05	94.5	94.5	bnlg1526- umc259	No

^aThe Institute for Genomic Research.
^bHTGS: the High Throughput Genomic Sequences.
^cyrGATE: Your Gene Structure Annotation Tool for Eukaryotes.
^dMap coordinate computed with MapMaker using 'RI self Haldane' options.
^eMap coordionate on REFMAP050110 obtained by homothetic projection with BioMercator [30].
Mapping of candidate genes was performed on the LHRF_Gnp2004 population derived from F2xF252 crossing, except for maize NCED6, ZEP1 and ZEP2 genes which were mapped on the IBM population (REFMAP050110 map) because of no polymorphism between F2 and F252 lines. QTL codes are detailed in Table 3.

ISSN: 1471-2229