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Table 3 Colocation between QTLs and candidate genes related to kernel desiccation and ABA biosynthesis

From: QTLs and candidate genes for desiccation and abscisic acid content in maize kernels

QTL name

Class

Bin

Position

From-to

Candidate gene

DW_80_2 ***

Growth

1.04

 

250-273

Rab17-EST (241)

FW_80_1 *

Desiccation

 

255

245-277

 

Water_60_1 *

Desiccation

 

333

305-344

NCED2(310)

%DW_60_2

Desiccation

1.06

338

323-386

Aquaporin (314)

AIP3 (323)

DW_80_3

Desiccation

 

460

455-464

 

FW_80_2 ***

Desiccation

1.08-1.09

460

457-463

NCED1 = Vp14(465)

Water_80_1 ***

Desiccation

 

460

455-463

 

ABA_embryo_30_1

ABA content

 

222

215-232

ZEP1(227)

ABA_embryo_40_2 ***

ABA content

2.04

226

219-234

 

ABA_endosperm_40_1 ***

ABA content

 

230

224-237

TIP2;1 (236)

Slope_1 **

Desiccation rate

 

237

235-241

 

Slope_3

Desiccation rate

3.05

220

212-244

Vp1 (209)

Slope_4

Desiccation rate

 

82

66-97

PM37 (82)

FW_30_1 *

Desiccation

4.03

92

79-109

 

FW_80_4

Desiccation

 

130

124-142

WSI724 (132)

Water_80_3 ***

Desiccation

4.04-4.05

132

124-132

 

%DW_30_2

Desiccation

4.06

173

161-189

PIP1;2 PIP1;4 (161)

ABA_endosperm_60_2

ABA content

4.10

340

329-346

ABI2 (334)

%DW_80_2 *

Desiccation

 

44

33-58

ZEP-EST(41)

DW_30_2

Growth

5.01

48

40-61

 

ABA_embryo_80_1

ABA content

 

96

77-115

CCD-EST (81)

%DW_60_4 *

Desiccation

5.02

102

83-122

Rab28 (132)

%DW_80_3

Desiccation

 

312

304-322

 

ABA_embryo_40_3 *

ABA content

5.07

 

302-322

NCED5(311)

DW_60_4

Growth

 

317

304-327

TIP2;2 (320)

%DW_60_5

Desiccation

6.05

148

136-175

Rab17-EST (138)

Water_60_4 **

Desiccation

  

136-201

Emb5 (138)

Water_60_5

Desiccation

 

167

163-173

PIP1;5 (164)

ABA_endosperm_30_2 **

ABA content

7.02

183

179-189

PIP2;1 (164)

PIP2;4 (164)

PIP2;6 (164)

ABA_endosperm_80_1

ABA content

7.03

244

236-288

PSY3 (241)

ABA_embryo_30_3

ABA content

7.05

383

365-402

Rehydrin (380)

Rate_30_40_2 ***

Desiccation rate

  

370-396

 

%DW_80_5 **

Desiccation

9.04

125

108-140

Rab17-EST (150)

ABA_embryo_60_1

ABA content

 

135

120-149

 

ABA_endosperm_80_2

ABA content

9.07

279

262-294

CCD-EST(271)

%DW_80_6 ****

Desiccation

 

291

269-314

 
  1. To compare with gene positions, QTLs mapped on the LHRF_F3:4 segregating population (Additional file 1) were projected on the REFMAP050110 map [32] using Biomercator [34]. Distances on REFMAP050110 are expressed in pcM and are shown in parentheses for gene candidates. Genes in bold were mapped by PCR in this study (Tables 4 and Additional file 1). Genes involved in kernel drying and located in the vicinity of the QTL confidence interval, are indicated in grey. ABI2: ABA-insensitive protein phosphatase 2C 2; AIP3: ABI3-interacting protein 3; Emb5: Embryogenic-ABA-inducible LEA 5; PM37: seed maturation protein; PSY3: phytoene synthase 3; Vp1: Viviparous1; WSI724: dehydrin. The asterisks behind each QTL represent the genomewide risk P (for definition see legend of Table 2) with * P < 0.25, ** P < 0.10, *** P < 0.05 and **** P < 0.01.