Transcript profiling of candidate genes related to water transfer, kernel maturation and ABA regulation. End point RT-PCR was performed on total RNA of the indicated tissues using gene-specific primers listed in Additional file 2. RNA quality and quantity were checked by total RNA loading on an agarose gel and ethidium bromide staining. The constitutively expressed 18 S rRNA gene was used as an internal control of RNA quantity. A, whole kernels without glumes at 30 to 80 DAP. B, Endosperms (End) and Embryos (Emb) at 30 to 60 DAP. The number of PCR cycles (end point) is indicated in brackets after the gene name.