Mutant alleles of FAD2-1A and FAD2-1Bcombine to produce soybeans with the high oleic acid seed oil trait

BMC Plant Biology

Table 1 Phenotype of different combinations of mutant FAD2-1A and mutant FAD2-1B produced in three environments.

Population			Oleic acid content (percent of total fatty acid)
	FAD2-1A	FAD2-1B	Costa Rica¹	Portageville, MO²	Columbia, MO³
2	Δ	P137R	81.4 ± 5.7^F5	82.2 ± 1.2^F7	79.1 ± 1.3^F8
3	Δ	I143T	80.0 ± 4.0^F5	65.0 ± 4.3^F7	58.7 ± 7.7^F8
4	S117N	P137R	81.1 ± 2.2^F2	81.7 ± 2.1^F3	77.3 ± 2.0^F3

¹Research station in Costa Rica. Seeds of F₅ generation of population of 2 and 3 were produced in winter 2006-2007, while F₂ seeds of population 4 were produced in winter 2008-2009. ² Plants were grown in Delta Research Center, seeds of F₇ generation of the populations 2 and 3 were produced in summer 2008 and F₃ generation of population 4 was produced in summer 2009. ³ All of the plants were grown summer 2009 at the Bradford Research & Extension Center, Columbia MO

ISSN: 1471-2229