Figure 5

Measurement of expression of six genes adjacent to the newly excised or inserted Dart -related TEs in two tissues (leaf and root) taken from the three rice- Zizania introgressant(s) and their rice parental line Matsumae under normal growing condition by real-time qRT-PCR analysis. (a) Diagrams showing the excision or insertion positions (vertical arrows) of Dart-related TEs in each of the four genes, and positions (horizontal arrowheads) of the gene-specific primers. The grey rectangles denote exons for each gene. (b) Amplification of the six genes on genomic DNA as templates (20 ng each sample) from the introgressants and their rice parental line Matsumae. The standard cultivar Nipponbare was also included as an additional control. The near identical amplifications indicate lack of amplification bias by the designed primers. (c) Transcriptional expression of each of the six genes in the leaf and root tissues taken from the introgressants and their rice parental line, measured by real-time qRT-PCR. The relative abundance of transcripts (mean ± SD) for each of the studied genes was calculated upon normalization against a rice β-actin gene (Genbank accession X79378). * and ** denote statistical significance at the 0.05 and 0.01 levels, respectively.