Figure 2

Mobilization of the Dart- related TEs in three rice- Zizania introgressants revealed by transposon-display (TD) and validated by locus-specific PCR amplification. (a) Exemplary profiles of Dart-specific TD. (b) Tabulated frequencies of excision and reinsertion of the element in each of the introgressants based on the TD-data. (c) Validation of the element transpositional activity by locus-specific PCR amplifications. The black and while triangles labelled in (a) denote excisions and reinsertions, respectively. Lanes 1 and 2 in (a) and (c) refer to TDs with the iDart1-51/nDart1-3 and iDart1s/nDart1-101s subgroup-specific primers (detailed in the manuscript text). The upper panel in (c) (from left to right) are examples of Dart-excisions that occurred in one (RZ2), two (RZ1 and RZ2) and all three (RZ1, RZ2 and RZ35) introgressants, which were detected with primers Dart-TDE-5, Dart-TDE-2 and Dart-TDE-3, respectively; the lower panel in (c) (from left to right) are examples of Dart-reinsertions that occurred in two (RZ1 and RZ2), one (RZ2) and one (RZ35) of the introgressants, respectively, which were detected with primers Dart-TDI-7, Dart-TDI-1 and Dart-TDI-27, respectively. Note that for a given locus, the fully sequenced standard rice cultivar Nipponbare, based on which the locus-specific primers were designed, may or may not contain the Dart copy.