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Table 2 Genes, primers, length of amplicons and amplification efficiency

From: Modulation of flavonoid biosynthetic pathway genes and anthocyanins due to virus infection in grapevine (Vitis viniferaL.) leaves

Gene1

Primer sequence 5'-3' (forward/reverse)

Amplicon length (bp)

qPCR efficiency

Reference

GenBank accession number*

a) Reference genes

Ubiquitin

TCTGAGGCTTCGTGGTGGTA/AGGCGTGCATAACATTTGCG

99

2.16

[82]

GU585868

Actin

CTTGCATCCCTCAGCACCTT/TCCTGTGGACAATGGATGGA

82

2.11

[56]

GU585869

GAPDH

TTCTCGTTGAGGGCTATTCCA/CCACAGACTTCATCGGTGACA

70

1.84

[56]

GU585870

EF1-a

GAACTGGGTGCTTGATAGGC/AACCAAAATATCCGGAGTAAAAGA

164

1.90

[56]

GU585871

SAND

CAACATCCTTTACCCATTGACAGA/GCATTTGATCCACTTGCAGATAAG

76

1.88

[56]

GU585872

NAD5

GATGCTTCTTGGGGCTTCTTGTT/CTCCAGTCACCAACATTGGCATAA

181

1.82

[83]

GU585873

b) Candidate genes

PAL

TCTGGTGGAAGGAATCCAAG/CAAAGTGCCACCAGGTAGGT

230

1.77

[62]

GU585850

CHS1

AGCCAGTGAAGCAGGTAGCC/GTGATCCGGAAGTAGTAAT

155

1.74

[61]

GU585851

CHS2

TCTGAGCGAGTATGGGAACA/AGGGTAGCTGCGTAGGTTGG

294

1.80

[61]

GU585852

CHS3

TCACTTGGACAGCCTTGTTG/CAATTCGAACATGGGCTTCT

106

1.87

$

GU585853

CHI1

CAGGCAACTCCATTCTTTTC/TTCTCTATCACTGCATTCCC

103

1.69

[84]

GU585854

CHI2

TCCAGATCAAGTTCACAGCA/GAAACAAGAGCCTCAAAGAA

127

1.60

[84]

GU585855

F3'H

ATTCGCCACCCTGAAATGAT/AGCCGTTGATCTCACAGCTC

196

1.82

[15]

GU585856

F3'5'H

GAAGTTCGACTGGTTATTAACAAAGAT/

AGGAGGAGTGCTTTAATGTTGGTA

156

1.68

[15]

GU585857

F3H1

CCAATCATAGCAGACTGTCC/TCAGAGGATACACGGTTGCC

69

1.83

[84]

GU585858

F3H2

CTGTGGTGAACTCCGACTGC/CAAATGTTATGGGCTCCTCC

129

1.70

[84]

GU585859

DFR

GAAACCTGTAGATGGCAAGA/GGCCAAATCAAACTACCAGA

114

1.85

[84]

GU585860

LDOX

AGGGAAGGGAAAACAAGTAG/ACTCTTTGGGGATTGACTGG

109

1.76

[84]

GU585861

UFGT

GGGATGGTAATGGCTGTGG/ACATGGGTGGAGAGTGAGTT

152

1.74

[84]

GU585862

MybA1

TAGTCACCACTTCAAAAAGG/GAATGTGTTTGGGGTTTATC

66

1.67

[84]

GU585863

FLS1

CAGGGCTTGCAGGTTTTTAG/GGGTCTTCTCCTTGTTCACG

154

1.82

[85]

GU585864

LAR1

AAATGAACTCGCATCTGTGT/CTGTGGGATGATGTTTTCTC

109

1.75

[82]

GU585865

LAR2

TGATATCAGCTGTGGGTGGA/CCCAAATTCTGATGGAAGGA

104

1.74

$

GU585866

ANR

GCTGCTGTTACCATCAATCA/GCAGGATAGCCCCAAGTAGG

113

1.62

[82]

GU585867

  1. 1See legends for Figure 1 and Figure 3 for names of genes
  2. *Accession numbers indicate sequences generated from this study.
  3. $Primers were designed based on sequence available in GenBank.