Sequence alignment of deduced PR10 proteins. The P-loop and Bet v 1 signature are framed. A star (*) marks the amino-acids implied in possible ribonucleasic activity (E102, E149 et Y151). Alignments were performed with ClustalW. A V. vinifera specific Bet v 1 motif was determined: G-[DG]-[VA]-L-x(4)-E-[SY]-[IL]-[CSATV]-[HY]-[ED]-x-[KST]-x-[VE]-x(3)-[GNDS]-G(2)-[CS]-x(2)-K-x(2)-[SK]-X-Y. In PR10.8 and PR10.10, the last aa varies in the P-loop motif (E and T, respectively); in PR10.9, E102 is replaced by D102, the 4th aa in the P-loop motif is E, and the Bet v 1 motif presents 4 differences (out of 34 aa) at the positions 1, 6, 23 and 29; in PR10.6, there is one difference (out of 33 aa) in the Bet v1 motif (position 12).