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Table 1 Average silent site divergence (K s ) and estimated age of split (bold) between M. truncatula and related species from the vicioide subclade, using three chloroplast gene regions: rbcL, matK and trnL

From: Medicago truncatula contains a second gene encoding a plastid located glutamine synthetase exclusively expressed in developing seeds

  Melilotus Trifolium Pisum Vicia
Medicago truncatula   rbcL   
  (0.0590) 9.1 My M. albus (0.0882) 13.6 My T. pratense (0.1194) 18.5 My P. sativum (0.1556) 24.1 My V. cracca
   matK   
  (0.0511) 6.7 My M. albus (0.0661) 8.7 My T. albopurpureum (0.1149) 15.2 My P. sativum (0.1103) 14.6 My V. hirsuta
   trnL   
  (0.0126) 6.6 My M. albus (0.0254) 13.2 My T. praetermissum (0.0211) 11.0 My P. sativum (0.0297) 15.5 My V. nipponica
Average Age of split 7.5 My 11.8 My 14.9 My 18.1 My
  1. As a calibration point we used the 34 million years estimate for the split between Pisum and Albizia species [27] and the levels of synonymous divergence per synonymous site (K s ) for the published chloroplast genes rbcL, matK and trnL of Pisum sativum and three Albizia species, namely the K s values of 0.2199 for P. sativum/A. julibrissin; 0.2575 for P. sativum/A. versicolor and 0.0653 for P. sativum/A. julibrissin, respectively.