The subcellular localisation of STR and SGD isolated from R. serpentina is conserved but the RsRGD does not aggregate in the nucleus. Undifferentiated C. roseus cells were co-transformed to express RsSTR, RsSGD and RsRGD GFP fusions (1st column) and a nucleus marker (2nd column). The merged image and the DIC morphology are presented in the 3rd and 4th columns, respectively. Note that both RsSTR and RsSGD displayed exactly the same fluorescence patterns as CrSTR and CrSGD, respectively. Note also that RsRGD-GFP and GFP-RsRGD displayed a diffuse nuclear fluorescence pattern reinforcing the specificity of the aggregation of CrSGD and RsSGD. Bar: 10 μm.