The C-terminal CrSGD bipartite nuclear localisation signal (NLS) is necessary and sufficient to drive the nuclear targeting of CrSGD and an accessible NLS-independent CrSGD sequence located upstream to the NLS promotes the aggregation of CrSGD. Undifferentiated C. roseus cells were co-transformed to express deletion/fusion forms of CrSGD (1st column) and organelle markers (2nd column). The merged image and the DIC morphology are presented in the 3rd and 4th columns, respectively. Note that the bipartite NLS from CrSGD was necessary for the nuclear targeting since fusion protein deprived from the NLS remained in the cytoplasm either displaying a diffuse pattern of fluorescence when the GFP was fused in the C-terminus extremity of the deleted CrSGD (a-d), or a punctuated (e-h) or fusiform (i-l) pattern of fluorescence when the deleted CrSGD C-terminus extremity was accessible in the fusion protein. Note also that the bipartite NLS was sufficient to drive the nuclear targeting but unable to promote the aggregation pattern (m-p). Bar: 10 μm.