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Table 3 Changes in the relative level of dlps in yellow lupin mitochondria.

From: Diverse accumulation of several dehydrin-like proteins in cauliflower (Brassica oleracea var. botrytis), Arabidopsis thaliana and yellow lupin (Lupinus luteus) mitochondria under cold and heat stress

Yellow lupin (Lupinus luteus)
Antibody K
Organ Imbibed seeds Hypocotyls
Stress
treatment
Size (kDa) Control Cold Heat Size (kDa) Control Cold Heat
  30 35.0 29.2 53.7 20 44.3 n.d. 115.0
  35 42.4 107.3 62.5 25 44.2 34.5 160.5
  40 63.3 177.0 131.5 35 54.3 120.3 85.0
  45 102.1 188.8 97.7 40 31.8 83.0 53.5
  55 171.5 183.5 144.3 45 n.d. 44.3 n.d.
  65/70 171.6 163.2 180.0 50/55 144.7 120.5 131.0
  80 n.d. 30.3 n.d. 65 187.5 123.0 133.3
      70 175.0 179.4 179.0
Antibody DHN24
Organ Imbibed seeds Hypocotyls
Stress
treatment
Size (kDa) Control Cold Heat Size (kDa) Control Cold Heat
  35 n.d. 18.3 n.d. 25 n.d. 43.1 n.d.
  40 12.5 27.2 16.8 40 25.3 47.4 n.d.
  50 40.6 108.9 39.4 50 121.9 148.8 79.0
  55 136.8 136.1 138.2 55 146.0 151.0 86.6
  65 87.6 123.7 78.9 65 117.8 132.3 39.7
  90 n.d. 34.1 10.3 70 41.7 58.0 23.4
  1. Thermostable proteins are denoted in bold. Thermolabile proteins (considered as not resistant for boiling in 15 min.) are underlined. n.d.- not detected or a very low- abundant protein. The thermostability of dlps of the lupin imbibed seed mitochondria was not determined. The results of densitometrical analysis of three independent Western blots were presented as average value in arbitrary units after background subtraction, as the optical density of protein bands per square pixel.