Skip to main content

Table 3 Changes in the relative level of dlps in yellow lupin mitochondria.

From: Diverse accumulation of several dehydrin-like proteins in cauliflower (Brassica oleracea var. botrytis), Arabidopsis thaliana and yellow lupin (Lupinus luteus) mitochondria under cold and heat stress

Yellow lupin (Lupinus luteus)

Antibody

K

Organ

Imbibed seeds

Hypocotyls

Stress

treatment

Size (kDa)

Control

Cold

Heat

Size (kDa)

Control

Cold

Heat

 

30

35.0

29.2

53.7

20

44.3

n.d.

115.0

 

35

42.4

107.3

62.5

25

44.2

34.5

160.5

 

40

63.3

177.0

131.5

35

54.3

120.3

85.0

 

45

102.1

188.8

97.7

40

31.8

83.0

53.5

 

55

171.5

183.5

144.3

45

n.d.

44.3

n.d.

 

65/70

171.6

163.2

180.0

50/55

144.7

120.5

131.0

 

80

n.d.

30.3

n.d.

65

187.5

123.0

133.3

     

70

175.0

179.4

179.0

Antibody

DHN24

Organ

Imbibed seeds

Hypocotyls

Stress

treatment

Size (kDa)

Control

Cold

Heat

Size (kDa)

Control

Cold

Heat

 

35

n.d.

18.3

n.d.

25

n.d.

43.1

n.d.

 

40

12.5

27.2

16.8

40

25.3

47.4

n.d.

 

50

40.6

108.9

39.4

50

121.9

148.8

79.0

 

55

136.8

136.1

138.2

55

146.0

151.0

86.6

 

65

87.6

123.7

78.9

65

117.8

132.3

39.7

 

90

n.d.

34.1

10.3

70

41.7

58.0

23.4

  1. Thermostable proteins are denoted in bold. Thermolabile proteins (considered as not resistant for boiling in 15 min.) are underlined. n.d.- not detected or a very low- abundant protein. The thermostability of dlps of the lupin imbibed seed mitochondria was not determined. The results of densitometrical analysis of three independent Western blots were presented as average value in arbitrary units after background subtraction, as the optical density of protein bands per square pixel.