Analysis of dehydrin-like proteins during pronase treatment of cauliflower mitochondria. The analysis was conducted at two temperatures (30°C and 37°C) and at three time points (10, 30 and 60 min.). C- control: cauliflower mitochondria untreated with pronase. Thirty micrograms of mitochondrial proteins were set per lane. Western analysis was carried out using the antibody against the K-segment of dehydrins (@K). Control antibodies directed against SHMT, VDAC-1 and the NAD9 subunit of complex I for the monitoring of the quality of cauliflower mitochondria during proteolytic digestion were additionally used (panels below). The representative Western blots came from 3 experimental repetitions.