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Figure 7 | BMC Plant Biology

Figure 7

From: Diverse accumulation of several dehydrin-like proteins in cauliflower (Brassica oleracea var. botrytis), Arabidopsis thaliana and yellow lupin (Lupinus luteus) mitochondria under cold and heat stress

Figure 7

Extraction of cauliflower mitochondrial dehydrin-like proteins by salt treatment. Dlps were extracted by KCl treatment in the case of unsonicated (A) and sonicated (B) cauliflower mitochondria. Cauliflower mitochondria (WM) were hypotonically lyzed and after the subsequent sonication (B) or without sonication (A) they were submitted to protein extraction at different concentrations of KCl (0, 0.25, 0.50, 0.75 M). After that all the samples were centrifuged and the supernatants (S) containing soluble proteins and pellets (P) enriched in membrane proteins were gel analyzed. Equal protein amounts by volume were set per lane (1/75). Western analysis was carried out using the antibody against the K-segment of dehydrins (@K). Control antibodies directed against SHMT, NAD9 subunit of complex I, CCMH protein, cyt. c and VDAC-1 for the monitoring of the purity of fractions were additionally used (panels below). The representative Western blots came from 3 experimental repetitions.

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