Figure 6
Western analysis of dehydrin- like proteins in cauliflower mitochondrial subfractions. Mitochondria from cauliflower inflorescences (1) were fractionated into fractions enriched in soluble matrix proteins (2), membrane proteins (3), peripheral membrane proteins (4) and in integral membrane proteins (5). Equal amounts of each subfraction volume were loaded onto the gel (ca. 1/75, 1/20 and 1/10). Peripheral and integral membrane proteins were loaded after acetone precipitation (lanes 4 and 5). Western analysis was carried out using the antibody against the K-segment of dehydrins (@K). Control antibodies directed against SHMT, NAD9 subunit of complex I, cyt. c1 and VDAC-1 for the monitoring of purity of fractions were additionally used (panels below). The representative Western blots came from 3 experimental repetitions.