Figure 5

Confocal imaging of N. tabacum pollen tubes co-expressing PIP5K4 with GFP-SYP124. Scale bars = 20 μm. A: Optical section of a non-growing pollen tube due to PIP5K over-expression. The cell exhibits partial loss of polarity and high protein localization in the flanks and apical plasma membrane. B: Optical section of a depolarized pollen tube due to PIP5K over-expression. SYP124 distribution is almost uniform in the swollen apex. C: Distribution of average fluorescence intensity along the first 40 μm of the pollen tubes plasma membrane (n = 10) as depicted in the diagram. The distribution is statistically different (P = 3E^-31) from GFP-SYP124 alone (compare with figure 1F). Error bars not displayed for the sake of clarity. D: Optical section of a halted pollen tube due to PIP5K over-expression. Along with high protein localization in the flanks and apical plasma membrane, labelling of large endomembrane-like structures is visible.