Figure 3

Time course analysis of GFP fluorescence intensity in N. tabacum growing pollen tubes transformed with GFP-SYPs (124, 121, 122) and exposed to 20 μM of extracellular Gd³⁺(A-C) or TMB-8 (D-F). Imaging and measurements were performed under the same conditions and settings as in figure 1E. Growth rate measurements (μm.min^-1) are displayed by the dashed lines. Addition of the inhibitors was performed at the time indicated by the vertical dotted line and collection of data resumed after inhibitor wash out. Each graph is representative of 5 independent pollen tubes. Symbols indicate measurements performed in the apical plasma membrane (black circle), apical cytoplasm (black square), sub-apical plasma membrane (lozenge) and sub-apical cytoplasm (triangle) according to the diagram in figure 1E.