Skip to main content
Figure 5 | BMC Plant Biology

Figure 5

From: Trichostatin A and 5-azacytidine both cause an increase in global histone H4 acetylation and a decrease in global DNA and H3K9 methylation during mitosis in maize

Figure 5

Reduction in H3K9me2 in TSA- and 5-AC treated cells. (A) Immunostaining for H3K9me2 in representative maize root tip cells in interphase or mitosis after TSA or 5-AC treatment. The 'DAPI' panel shows DAPI-stained DNA images, the 'H3K9me2' panel shows immunostained images and the 'Merge' panel shows a combination of blue and green signals. Dispersed H3K9me2 staining was observed throughout interphase and mitosis in the control cells. All TSA- and 5-AC-treated cells during interphase and mitosis have lower H3K9me2 levels than the untreated control cells. Bar = 5 μm. (B) Histogram showing mean gray values of the immunostaining signals for H3K9me2. The mean gray value for H3K9me2 after treatment with TSA or 5-AC is lower than that of the control. The mean gray values for H3K9me2 were derived from Additional file 1: Table S2. Error bars represent standard error of the mean.

Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us