Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging

Takahashi, Akira; Hayashi, Nagao; Miyao, Akio; Hirochika, Hirohiko

doi:10.1186/1471-2229-10-175

BMC Plant Biology

Table 1 Allele of pish(t) obtained in this screening

From: Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging

	Line Name	Mutation	Direction^a)	Position^b)	Origin^c)
1	NC7869	Tos17 insertion	↑	+2.4	N0
2	NC8589	Tos17 insertion	↑	+2.2	N0
3	ND0085	Tos17 insertion	↓	+1.1	N1
4	ND2294	Tos17 insertion	↓	+2.8	N3
5	ND2607	Tos17 insertion	↓	+1.7	N3
6	ND2794	Tos17 insertion	↓	+3.6	N3
7	ND3377	Tos17 insertion	↑	+2.4	N4
8	ND3760	Tos17 insertion	↑	+1.5	N4
9	ND5163	Tos17 insertion	↑	+1.1	N7
10	ND5216/ND5490/ND5546	Tos17 insertion	↑	+1.3	N7
11	ND5907	Tos17 insertion	↑	+1.0	N7
12	ND6103	Tos17 insertion	↓	+2.7	N8
13	ND6886	Tos17 insertion	↑	+1.0	N8
14	ND7244/ND7372	Tos17 insertion	↑	+0.4	N9
15	ND7350/ND7813	Tos17 insertion	↓	-0.4	N9
16	ND7933	Tos17 insertion	↓	+3.7	N9
17	ND9375	Tos17 insertion	↓	+2.9	N12
18	ND9468	Tos17 insertion	↓	+1.3	N12
19	NE0979	Tos17 insertion	↓	+0.7	N13
20	NE1033	Tos17 insertion	↑	+3.5	N14
21	NE1136	Tos17 insertion	↓	+0.1	N14
22	NE1348	Tos17 insertion	↓	+2.4	N14
23	NE1445	Tos17 insertion	↑	+3.3	N14
24	NE1544/NE1588/NE1602/NE1617	Tos17 insertion	↓	+2.1	N15
25	NE1662	Tos17 insertion	↓	+1.0	N15
26	NE1710/NE1864	Tos17 insertion	↓	+2.4	N15
27	NE2127	Tos17 insertion	↓	+3.4	N16
28	NE3788	Tos17 insertion	↑	-0.9	N18
29	NE3814	Tos17 insertion	↑	+2.3	N18
30	NE9963	Tos17 insertion	↓	+3.4	N19
31	NF1237	Tos17 insertion	↑	+3.4	N28
32	NF1437/NF1457	Tos17 insertion	↓	+0.6	N28
33	NF2942	Tos17 insertion	↑	+3.6	N39
34	NF6474	Tos17 insertion	↑	+2.4	N33
35	NF6868	Tos17 insertion	↓	+2.0	N44
36	NF7813	Tos17 insertion	↑	+1.4	N42
37	NF7959	Tos17 insertion	↓	+2.2	N42
38	NG0430	Tos17 insertion	↑	+1.9	N47-2
39	NG2085	Tos17 insertion	↑	+1.7	N51
40	NG3372	Tos17 insertion	↑	+2.8	N54
41	NG3664	Tos17 insertion	↓	+2.8	N55
42	NG5334	Tos17 insertion	↑	-2.4	N61
43	NG6314	Tos17 insertion	↓	+3.9	N65
44	NG7885	Tos17 insertion	↑	+2.6	N68
45	NG8190	Tos17 insertion	↓	+3.3	N69
46	NG8193	Tos17 insertion	↓	+2.3	N69
47	NC7524/NC8928	Large deletion			N0
48	NC7925	Large deletion			N0
49	ND2043	Large deletion			N3
50	ND2602/ND2810	330bp deletion			N3
51	ND6227	Large deletion			N8
52	ND8614	Large deletion			N11
53	NE2386	24bp deletion			N16
54	NF4922/NF4946/NF4950	56bp deletion			N41
55	NG2263	Large deletion			N52
56	ND2032/ND2105/ND2452/ND2562	Unknown insertion			N3

a) Downward arrows indicate insertions with Tos17 oriented in the same direction as Pish and the Npi37-1 to Npi37-3 genes. Upward arrows indicate insertions in the opposite orientation.
b) Positions (kb) of Tos17 insertions relative to the Pish ATG initiation codon.
c) Name of the flasks in which their origin calli were cultured.

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ISSN: 1471-2229

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