Expression of AtNudt7 and its closest homologs in WT Col-0 plants growing in different potting mixes. A. Gene expression levels of AtNudt2, AtNudt6 and AtNudt7 in WT Col-0 plants grown in 12:3:1 and Metro-Mix 200 (MM) potting mix, as determined by real-time PCR. Fold change in 12:3:1 mix was plotted with respect to expression in MM. Error bars indicate standard deviation of three replicates. Bars with different letters indicate statistically significant differences (p-value < 0.05) in gene expression in WT plants grown in 12:3:1 mix when compared with those grown on MM. B. Detection of AtNUDT7 protein in WT Col-0 plants grown in the 12:3:1 potting mix and Metro-Mix 200 (MM) using AtNUDT7 polyclonal antibodies.