Induction of LOX2 and VSP2 in response to K deficiency. Quantitative PCR analysis of LOX2 (squares) and VSP2 (triangles) in shoots A. thaliana plants grown from germination for 12 to 16 days on vertical Petri dishes containing control medium (C, black symbols) or K-free medium (-K, open symbols). Approximately 50 plants were pooled for RNA isolation. Bars show averages ± standard error of technical replicates, normalised to the expression level of the constitutive gene YLS8 (see Material and Methods for qPCR details and primers). Invisible error bars are smaller than symbols.