Figure 2

The stunted growth phenotype of stably transformed IR _α-dox S plants is associated with a stronger silencing of Na α-dox1 and a co-silencing of Na α-dox2. Transcript accumulation (mean ± SE of 5 biological replicates) as measured by SYBR Green-based real time-PCR of (A) Naα-dox1 and (B) Naα-dox2 in wild-type Nicotiana attenuata (WT) and plants transformed with an inverted repeat (IR) construct to silence Naα-dox1. The transcript abundance is expressed relative to WT expression. Whereas lines that are morphologically similar to WT plants (IR _α-dox M) show a moderate silencing of α-dox1, severely stunted lines (IR _α-dox S) are more strongly reduced in α-dox1 transcripts and co-silenced for the α-dox2 gene. Leaves had been wounded and treated with 2.5 μL of OS every 30 min for four times. Leaves were harvested 30 min after the last treatment. Asterisks signify significant differences between IR _α-dox and WT plants (unpaired t-test for transcripts of Naα-dox1: M1 P = 0.04, M2 P = 0.03, S1 P = 0.001, S2 P = 0.01; of Naα-dox2: M1 P = 0.37, M2 P = 0.63, S1 P = 0.01, S2 P = 0.03).