Northern analysis of the transcript levels of 35S: AOP2
in the transformed lines. Leaf tissue from five T2 plants from each independent 35S:AOP2
line was pooled and total RNA was extracted. Lane 1 contains total RNA extracted from the leaf tissue of five Cvi plants, which express AOP2, and was included as a positive control. Lane 2 contains total RNA extracted from leaf tissue of five wild-type Col plants, and was included as a negative control. Lanes 3 - 12 contains total RNA from the transgenic lines indicated and shows the transcript levels of AOP2 in the ten independently transformed lines. The upper panel depicts 28S rRNA stained with ethidium bromide to indicate the level of total RNA loaded in each lane.