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Figure 6 | BMC Plant Biology

Figure 6

From: The characterisation of AOP2: a gene associated with the biosynthesis of aliphatic alkenyl glucosinolates in Arabidopsis thaliana

Figure 6

Gel electrophoresis of PCR-amplified fragments encompassing AOP2 and AOP3 promoter regions and the structural genes. PCR reactions, using genomic DNA from the Col, Pi and Ler as template, were performed with AOP2 and AOP3 gene-specific primers designed to hybridize at flanking positions in promoter regions and CDS regions approximately 300 bp distal from the ATG translation initiation codon. Lanes 1,5 & 9; products obtained from AOP2 promoter and CDS primers, Lanes 2, 6 & 10; products obtained from AOP3 promoter and CDS primers, Lanes 3, 7 & 11; products obtained from AOP2 promoter and AOP3 CDS primers, Lanes 4, 8 & 12; products obtained from AOP3 promoter and AOP2 CDS primers. Lane M contains λ/BstII size markers.

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