PCR amplification of VRN1 promoter region with primers AP1_ProDel_F1/AP1_ProDel_R1. A. Diploid wheat accessions. T_b - T. boeoticum, T_m - T. monococcum, Ae_sp - Ae. speltoides, Ae_sq - Ae. squarrosa. T_b PI-427328, K-20741, K-40118 have 20 bp deletion. W - winter growth habit. B. Spring diploid Triticum and Aegilops accessions. T_b IG-45296 has 20 bp deletion, T_m K-18105 has 34 bp deletion. M - Marker-Bioline HyperLader IV.