Figure 2

Gel electrophoresis images of cDNA from the apical and basal cells of tobacco two-celled proembryo by LD-PCR and products of PCR-select cDNA subtraction. (a) cDNAs synthesized from the apical cell (AC) and basal cell (BC). M, Molecular marker 1 kb DNA ladder. (b) Products of PCR-select cDNA subtraction. M, Molecular marker DL2000; SA, the second PCR product of the apical cell/basal cell subtraction; UA, the second PCR product of the unsubtracted control; SB, the second PCR product of the basal cell/apical cell subtraction; UB, the second PCR product of the unsubtracted control.