Development of Efficient Plant Regeneration and Transformation System for Impatiens Using Agrobacterium tumefaciens and Multiple Bud Cultures as Explants

Table 1 Mean percent of explants producing shoots of I. walleriana cvs. Accent Red and Salmon Picote after cotyledonary nodes were placed on medium with four different concentrations of TDZ 4 weeks after culture.

Cultivar	TDZ concentration (μM)	No. of explants	% of explants producing shoots^x (mean ± standard error)
Accent Red	1	20	40.0 ± 9.4 A
	3	30	26.7 ± 7.7 AB
	5	30	6.7 ± 7.7 B
	7	20	35.0 ± 9.4 A
Salmon Picote	1	26	50.0 ± 8.2 A
	3	30	10.0 ± 7.7 B
	5	30	26.7 ± 7.7 B
	7	22	18.2 ± 9.0 B

^x Treatments followed by different letters are significantly (P < 0.05) different using the LSD All-Pairwise Comparison Test (Statistix 9, Analytical Software, Tallahassee, FL, USA).