Figure 2

Effect of Z-Asp-CH2-DCB on DNA fragmentation in xylogenic Zinnia cell cultures. The cells were harvested 120 h after the induction of differentiation. DNA was isolated, separated and stained with ethidium bromide as described in Material and Methods. M, marker 1 kb; lane 1 (c - h), negative control of non-induced cells; lane 2 (c + h), 1 mg/L NAA + 1 mg/L BA; lane 3, 1 mg/L NAA + 1 mg/L BA + 100 nM of the of the irreversible broad spectrum caspase inhibitor Z-Asp-CH2-DCB.