A. Multiple sequence alignment of At FYVE domains. Absolutely and moderately conserved residues are highlighted in black and grey, respectively. For comparison, the secondary structures of EEA1 FYVE domain (PDB: 1JOC chain A ) are highlighted in red (α-helix) and blue (β-sheet). All eight cysteine residues are colored yellow. B. A schematic localization of AtFYVE domain-containing proteins within the Arabidopsis genome.