|
Substrate
|
Km(μM)
|
Vmax(pkat/mg)
|
Kcat/Km(nM-1. s-1)
|
|---|
|
Wild type
| | | |
|
Tricetin
|
59.51 ± 0.79
|
110 ± 7.0
|
74
|
|
5HFA
|
270.98 ± 11.51
|
210 ± 6.0
|
31
|
|
V309I
| | | |
|
Tricetin
|
101.08 ± 9.68
|
35 ± 8.0
|
13
|
|
5HFA
|
18.24 ± 1.72
|
59 ± 2.0
|
130
|
- aThe affinity purified recombinant proteins (1.8 μg) were incubated with 5.0 to 80 μM of the indicated substrates, 1 mM of AdoMet containing 125 nCi of the [3H] label for 15 min at 30°C, and the activity in the products was determined as described in the Methods section. The data are averages of three determinations ± SE; pkat, the catalytic activity that raises the reaction rate by one pmol.s-1.
