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Table 3 Kinetic parameters of wild type TaOMT2 and mutant V309I for tricetin and 5HFA as substratesa

From: Structure-function relationships of wheat flavone O-methyltransferase: Homology modeling and site-directed mutagenesis

Substrate

Km(μM)

Vmax(pkat/mg)

Kcat/Km(nM-1. s-1)

Wild type

   

Tricetin

59.51 ± 0.79

110 ± 7.0

74

5HFA

270.98 ± 11.51

210 ± 6.0

31

V309I

   

Tricetin

101.08 ± 9.68

35 ± 8.0

13

5HFA

18.24 ± 1.72

59 ± 2.0

130

  1. aThe affinity purified recombinant proteins (1.8 μg) were incubated with 5.0 to 80 μM of the indicated substrates, 1 mM of AdoMet containing 125 nCi of the [3H] label for 15 min at 30°C, and the activity in the products was determined as described in the Methods section. The data are averages of three determinations ± SE; pkat, the catalytic activity that raises the reaction rate by one pmol.s-1.