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Table 3 Kinetic parameters of wild type TaOMT2 and mutant V309I for tricetin and 5HFA as substratesa

From: Structure-function relationships of wheat flavone O-methyltransferase: Homology modeling and site-directed mutagenesis

Substrate Km(μM) Vmax(pkat/mg) Kcat/Km(nM-1. s-1)
Wild type    
Tricetin 59.51 ± 0.79 110 ± 7.0 74
5HFA 270.98 ± 11.51 210 ± 6.0 31
V309I    
Tricetin 101.08 ± 9.68 35 ± 8.0 13
5HFA 18.24 ± 1.72 59 ± 2.0 130
  1. aThe affinity purified recombinant proteins (1.8 μg) were incubated with 5.0 to 80 μM of the indicated substrates, 1 mM of AdoMet containing 125 nCi of the [3H] label for 15 min at 30°C, and the activity in the products was determined as described in the Methods section. The data are averages of three determinations ± SE; pkat, the catalytic activity that raises the reaction rate by one pmol.s-1.