Figure 5
dCAPS and indel analyses of an F 2 population derived from a cross between a soybean near-isogenic line with white flowers, Clark- w1 and a Glycine soja line with light purple flowers, B09121. (Upper panel) Results of dCAPS analysis. PCR products with dCAPS primers were digested with NcoI and the digests were separated on an 8% polyacrylamide gel. Ï•, molecular marker Ï•x174/HaeIII; clk, Clark; w1, Clark-w1; B, B09121; W, F2 plants homozygous for w1 allele (white flower); Lp, F2 plants homozygous for w1-lp allele (light purple flower); H, heterozygous F2 plants (light purple flower); P, an F2 plant with purple flowers. The migration of size markers is shown to the left of the gel. (Lower panel) Results of indel analysis. PCR products amplified with indel primers were separated on an 8% polyacrylamide gel.