Figure 3
HPLC chromatogram of anthocyanins extracted from flower petals of PI 424008A (upper panel) and B09121 (lower panel). 200 mg of banner petals was extracted with 2 ml of MeOH containing 0.1% HCl. Eluents: MeCN/HOAc/H2O/H3BO3 (6:8:83:3). Flow-rate: 1.0 ml/min. Injection: 10 μl. Detection: 530 nm, A1 = malvidin 3,5-di-O-glucoside, A2 = petunidin 3,5-di-O-glucoside, A3 = delphinidin 3,5-di-O-glucoside, A4 = delphinidin 3-O-glucoside, A5 = peonidin 3,5-di-O-glucoside, A6 = cyanidin 3,5-di-O-glucoside, A7 = cyanidin 3-O-glucoside.