Amino acid sequence of F3'5'H gene from Glycine soja line B09121 with light purple flowers and schematic diagram of dCAPS analysis to detect a base substitution found in B09121. (A) Amino acid sequence of F3'5'H gene from Glycine soja line B09121 with light purple flowers. Amino acid position 210 (underlined) was methionine (valine in Clark) and amino acid position 475 (underlined) was valine (glutamic acid in Clark). (B) Schematic diagram of dCAPS analysis to detect a single-base substitution found in B09121 (shown in bold). NcoI site (CCATGG, double underlined) was artificially introduced in the PCR products of Clark using a forward primer with a mismatched base C that is exhibited in bold italic. Annealing sites of PCR primers are underlined. PCR product is digested by NcoI only in Clark generating size differences of 18 bp.