EBR effects on inhibition of germination by NaCl in WT and mutant seedlings. (A) WT (Col), ein2, eto1-1 and npr1-1 were allowed to germinate on a nutrient medium containing 150 mM NaCl in the absence (C) or presence of 1 μM EBR (E). Photographs of the seedlings were taken 3 days after imbibition. (B) Percentage of seeds germinated was calculated by counting the number of seedlings with emerged cotyledons at 3 days after imbibition. (C) Percentage of seedlings surviving on 150 mM NaCl was calculated by counting the number of seedlings that showed true leaves and green colour at 20 days after imbibition. All experiments were performed in triplicates with n > 30. Error bars represent SE of mean for three replicates. Asterisks above the histograms indicate the significance of differences between untreated and EBR-treated genotypes as analyzed by Student's t-test (**p < 0.01).