Figure 3

Effect of EBR on the expression of SA, JA/ET and ABA response genes. Total RNA was isolated from WT and mutant seedlings grown in the absence (C) or presence (E) of EBR at 22°C, and transcript levels were analyzed by RT-PCR. Actin was included as a control for constitutive expression. (A-D) Expression profiles of PR-1, WRKY70, and WAK1 in WT and SA-related genotypes; PDF1.2 and LOX2 in WT and JA mutants; PDF1.2 and HEL in ET-related genotypes; LTP4 and RD22 in ABA mutants. (E) Expression profile of the stress-responsive GST1 in WT (Col) and SA genotypes. (F) Down-regulation by EBR of DWF4 in WT (Col) and SA-insensitive npr1-1 mutant.