Table 4 Characteristics of gene specific real-time RT-PCR assays
Gene symbol | Gene name | Accession No. | Primer sequence (5'→3') | Amplicon size | PCR efficiency |
|---|---|---|---|---|---|
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | AK251456 | Forward: TGTCCATGCCATGACTGCAA | 105 | 101% |
Reverse: CCAGTGCTGCTTGGAATGATG | |||||
TUBA | Alpha tubulin-2B | AK250165 | Forward: TTCGCCCGTGGTCATTACA | 113 | 100% |
Reverse: GCATTGAAGACAAGGAAGCCC | |||||
TUBB | Beta-tubulin | U76897 | Forward: CAAGGAGGTGGACGAGCAGATG | 84 | 97% |
Reverse: GACTTGACGTTGTTGGGGATCCA | |||||
ELF1A | Elongation factor-1 alpha | AF479046 | Forward: CAGTGCTGGACTGCCACA | 164 | 91% |
Reverse: CTCCACCACCATGGGCTT | |||||
EIF4A | Eukaryotic initiation factor 4a | EU850433 | Forward: TTGTGCTGGATGAAGCTGATG | 76 | 99% |
Reverse: ACACCAACAGCCACAGTTTGC | |||||
18S rRNA | 18S ribosomal RNA | M82356 | Forward: GTGACGGGTGACGGAGAATT | 151 | 85%, 99%* |
Reverse: GACACTAATGCGCCCGGTAT | |||||
28s rRNA | 28S ribosomal RNA | M82206 | Forward: CCTGATCTTCTGTGAAGGGTTCGA | 172 | 94% |
Reverse: GGTTCGATTAGTCTTTCGCCCCTA | |||||
BYDV cp | coat protein of BYDV | EF521849 | Reverse:TGTTGAGGAGTCTACCTATTTG | 294 | 99% |
Forward:GTTGAGTTTAAGTCACACGC |