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Table 3 Ranking of candidate reference genes and choice of best pair of reference genes by NormFinder and GeNorm tools

From: Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR

gene

GeNorm stability value (M)

  

NormFinder stability value

 

wheat

barley

oat

all

wheat

barley

oat

all

 

GAPDH

1,09

1,06

1,08

2,0

0,088

0,112

0,035

0,074

 

TUBB

1,14

1,27

1,12

2,0

0,041

0,085

0,108

0,098

 

TUBA

2,13

1,54

1,59

4,43

0,785

0,201

0,205

0,289

 

18S rRNA

1,48

1,27

1,14

2,15

0,076

0,158

0,125

0,248

 

28S rRNA

1,82

1,71

1,90

2,78

0,378

0,354

0,258

0,487

 

EIF4a

1,48

2,81

2,59

2,82

0,352

0,489

0,301

0,499

 

EEF1A

2,08

2,48

2,49

2,87

0,181

0,187

0,355

0,241

 
  1. The greater expression of stability is indicated by a lower stability value (M). GeNorm stability is based on an estimate of the pairwise variation (M). For NormFinder analysis, samples were grouped into healthy, abiotic stress, and virus-infected groups. The stability is calculated from the intra- and inter-group variation and the best combination of genes is also given.