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Table 3 Ranking of candidate reference genes and choice of best pair of reference genes by NormFinder and GeNorm tools

From: Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR

gene GeNorm stability value (M)    NormFinder stability value
  wheat barley oat all wheat barley oat all  
GAPDH 1,09 1,06 1,08 2,0 0,088 0,112 0,035 0,074  
TUBB 1,14 1,27 1,12 2,0 0,041 0,085 0,108 0,098  
TUBA 2,13 1,54 1,59 4,43 0,785 0,201 0,205 0,289  
18S rRNA 1,48 1,27 1,14 2,15 0,076 0,158 0,125 0,248  
28S rRNA 1,82 1,71 1,90 2,78 0,378 0,354 0,258 0,487  
EIF4a 1,48 2,81 2,59 2,82 0,352 0,489 0,301 0,499  
EEF1A 2,08 2,48 2,49 2,87 0,181 0,187 0,355 0,241  
  1. The greater expression of stability is indicated by a lower stability value (M). GeNorm stability is based on an estimate of the pairwise variation (M). For NormFinder analysis, samples were grouped into healthy, abiotic stress, and virus-infected groups. The stability is calculated from the intra- and inter-group variation and the best combination of genes is also given.