Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR

Table 2 Results of Bonferroni tests

Gene	Healthy vs. Abiotic stress			Healthy vs. Virus Infected			Abiotic stress vs. Virus infected
	barley	oat	wheat	barley	oat	wheat	barley	oat	wheat
28S rRNA	ns	ns	ns	ns	ns	ns	ns	ns	ns
18S rRNA	***	***	***	***	ns	ns	ns	*	***
TUBA	ns	ns	***	ns	ns	**	ns	ns	***
TUBB	ns	ns	ns	ns	ns	**	ns	ns	***
EIF4A	ns	ns	ns	ns	ns	ns	ns	ns	ns
EF1A	ns	ns	ns	ns	ns	ns	ns	ns	ns
GAPDH	ns	ns	***	ns	ns	***	ns	ns	***

Results of Bonferroni tests applied on the data to compare the value of each column (factor 'fitness' - healthy; biotic stress, or abiotic stress) and each row (factor 'species').
P > 0.05 considered as non significant (ns), P < 0.01 as significant (*), P < 0.001 as very significant (**), and P < 0.0001 as extremely significant (***).

ISSN: 1471-2229