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Figure 4 | BMC Plant Biology

Figure 4

From: Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR

Figure 4

Titre of BYDV in two oat varieties as determined by three quantification approaches. (A): The relative BYDV titre levels in all oat samples determined by relative quantification using GAPDH, TUBB, TUBA and 18S rRNA (GAPDH group); 28S rRNA, EIF4A and EF1A (EIF4A group); and by absolute quantification with cloned standards (absolute). The absolute numbers were transformed into relative ratios for comparison. The boxes represent the lower and upper quartiles with medians; the whiskers illustrate the 10-90 percentiles of the samples. (B): The relative BYDV titre levels in the two oat cvs. Atego (tolerant) and Saul (susceptible) determined by relative quantification using GAPDH, TUBB, TUBA and 18S rRNA (GAPDH group); 28S rRNA, EIF4A and EF1A (EIF4A group); and by absolute quantification with cloned standards (absolute). The absolute numbers were transformed into relative ratios for comparison. No significant differences were recorded.

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