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Figure 1 | BMC Plant Biology

Figure 1

From: Increasing the amylose content of durum wheat through silencing of the SBEIIagenes

Figure 1

Semiquantitative RT-PCR of transcripts encoding starch biosynthetic enzymes. 1.5% agarose gel of specific amplicons of starch genes in total RNA of wheat endosperm collected at 18 dpa. 1) cv Svevo, and the corresponding SBEIIa RNAi lines: 2) MJ16-112; 3) MJ16-114; 4) MJ16-119. 5) cv Ofanto, and the corresponding SBEIIa RNAi lines: 6) A428, 7) A431; 8) A432. Gadph was used as housekeeping gene. SbeIIa, Starch branching enzyme IIa; GbssI, Granule bound starch synthase I; SsI, Starch synthase I; SsII, Starch synthase II; SsIII, Starch synthase III; SbeI, Starch branching enzyme I; SbeIIb, Starch branching enzyme IIb; LD, Limit dextrinase; Isa1, Isoamylase 1; Gadhp, glyceraldehyde-3-phosphate dehydrogenase.

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