Figure 2

Prediction of gene structures in PIP, TIP and NIP subgroups of cotton aquaporin genes. Open box: exons. Black line: introns. Black shading: Two NPA motifs in loop B and loop E. A, Sequence identity of exons and introns in same position is shown as a percentage. Nucleotide similarity between same groups is indicated in solid lines and dotted lines identify similarity between different groups. B, Additional exon-intron structures are shown for TIP1;15, NIP1;1 and NIP6;1 that have no homologous genomic sequences available for comparison. NIP6;1 is partial at the C-terminal end. Positions of degenerate primer pairs used for amplifying genomic fragments are marked as half arrows.