Figure 1

Phylogenetic analysis of 71 members of the cotton aquaporin family with members of other plants. Deduced amino acid sequences were aligned using ClustalW2 and the phylogenetic tree was generated using Bootstrap N-J tree (1,000 resamplings) method and TreeView program (v1.6.6). The name of each group and subgroup is indicated next to the corresponding group. The distance scale denotes the number of amino acid substitutions per site. Gr, Gossypium raimondii; At, Arabidopsis thaliana; Vv, Vitis vinifera; Os, Oryza sativa; Pp, Physcomitrella patens; Pt; Populus trichocarpa; Rc, Ricinus communis; Nb, Nicotiana benthamiana; Sl, Solanum lycopersicum. Bold gene names with no species initials are for cotton aquaporins. Asterisks denote genes with partial sequence. Only 63 cotton aquaporins were included here among those identified in this study because eight of the aquaporin transcripts encode proteins of identical sequence resulting in only 63 unique protein sequences.