Table 1 The primers of genes detected by RT-PCR and real-time PCR analyses.
Putative Genesa(Accession No.) | RT-PCR primers (5'-3') | Real-Time PCR primers (5'-3') |
|---|---|---|
WIP (GO349116) | F:GACATCGTATCATCAGGAAAAG | F: CGTATCATCAGGAAAAGTTGG |
| Â | R: CTCTCACCTGGGTAATTATC | R: GCTAGAATCGCAGCCTTCAG |
SAP (GO349117) | F: GAATACGAACCGTGAAAGCG | F: GTGGCCTATCGATCCTTTAG |
| Â | R: CTAGGTTGAATTACCATCGC | R: ACGTCGCTATGAACGCTTGG |
HSP (GO349120) | F: TTGTCCCTTAGAGCTTGTG | F: GTTGTCCCTTAGAGCTTGTG |
| Â | R: CTCCACCAAGAGTGGTGATG | R: GACCGTGTTGTTGATTCTCC |
MT (GO349141) | F: ACCAAGGCAGAACTGAAGG | F: CACCAAGGCAGAACTGAAGG |
| Â | R: CCACTTTCTGATTCCTCCTC | R: CTGGATGTAGGATTCATCGG |
CP450 (GO349147) | F: GACAAGGGGAATCCGACTG | F: CAATGTGATTTCTGCCCAGTG |
| Â | R: CCACACGAGATTTCTGTTCTC | R: TGACGAGGCATTTGGCTACC |
Cullin (GO349159) | F: GTCCAATTGGTAATGCTTAAAC | F: CCTGCTCAAGCTATCCCTTC |
| Â | R:GGAAAGAGCCCATGAGTAAAG | R: GTCACGATTTCTGGCCAACC |
UL (GO349162) | F: AGGATTAGACTTGACATAGCG | F: GATGGCCGATGGTTGAAAATG |
| Â | R: CTCCAATTTATCAGTGCCGC | R: CTGGATCTGAGTTTGCTGTTG |
PPA (GO349189) | F: GTTGAACCGGAGGAATTTCC | F: GAATCGGATTCGTGGAAGTG |
| Â | R: TCATGGCCAGAAATGTCTCC | R: CTGTCCCTCAATCTCAAAAGG |
LPH (GO349193) | F: TGTGCCACCAAGGTGCAAAC | F: GCAAGGGATCCAGGTTCATC |
| Â | R: GTCGTGGCAAACATACGTTG | R: CACCTTATGTGCGACATCGAC |
DBP (GO349205) | F: TGAACTGGAACAGAGCAAGC | F:GATGAACTGGAACAGAGCAAG |
| Â | R: TTGACCCCGAACAATCTCAG | R: CTGTTCACGTTCTTTCAGAAC |
TCTP (GO349268) | F: GCCTCCATCAGCGTTTTCAG | F: GTGTCAACAACTTGATGAACC |
| Â | R: CCCTCAATGATATCGACACC | R: ATGAGGGTGTTGATGACCAG |
SRPP (GO349270) | F: CTGAAGAGGTGGAGGAAGAG | F: CCTTTATGCCAAGGACATATC |
| Â | R: CAGAGCTTTTGCGCCTTCCT | R: GTCTACAAACTTGACAGCCTC |
AGPS (GO349278) | F: TTCTCACTCTCTCACGATGG | F: CCATACAATCTTACACTCACC |
| Â | R: CGAGGTTCATTCCACACATC | R: CTATGTCCAGCTCGTCCTTC |
HH2A (GO349279) | F: AAGAATTCCGCGGCCTCCTG | F: GAAGCCAGTTTCTAGGTCTG |
| Â | R:ACTCGAGAACGGCAGCCAAG | R: GGCAGCCAAGTAAACTGGAG |
CT (GO349296) | F: ATCCTTCTCCTGATCTCCTC | F: TTCTCCTGATCTCCTCCAAG |
| Â | R: GGGAGCGAGAGGAAGCTAG | R: CAAACAAGAGCGTTACCTCG |
GR (GO349295) | F: TGAGGCATTGAGTCTGGAAG | F: TGAGGCATTGAGTCTGGAAG |
| Â | R: TGCTCTATTGCCTGCTCTTC | R: GAAAACTAGGTCCACAGTGG |
VPSAP (GO349303) | F: ATTGTATGAGGACTCGAGATC | F: GAGGACTCGAGATCAAAGTG |
| Â | R:AGAACTTTGCTGAGCTCTATG | R:GAAGACAAAGATGAAAGAGTGG |
HbMyb1 (GO349308) | F: AGGATGAACCTGATCGATGG | F: CCAGACACCAAGTCTCCTTC |
| Â | R: CTTCTCTCATCCTTCCCTCC | R: ATCCTTTGGCCATGCCAACC |
CHP (GO349321) | F:CTCAACAACTCCAGTTGGTG | F: TCTTCAAAGCTCAACAACTCC |
| Â | R: ACCACTTCGACATATCCTCC | R: TTGCAGGCTCTCAATTGCTC |
Y17H05 (GO349330) | F:GTGATGCATGATGTGAGGAG | F: TGGTGATGCATGATGTGAGG |
| Â | R:GAAATCCTAGGGCATCATTG | R: GTAAGACCATTGGACAAACGG |
18s rRNA gene (AB268099) | F: GGTCGCAAGGCTGAAACT | F: GCTCGAAGACGATCAGATACC |
| Â | R: ACGGGCGGTGTGTACAAA | R: TTCAGCCTTGCGACCATAC |