The expression analyses of differently expressed genes by RT-PCR and real-time PCR. Total RNA extracted from the latex was used for RT-PCR and real-time PCR analyses with the 18s rRNA as the internal reference. H and T represent healthy and TPD trees, respectively. The gene-specific primers were provided in Table 1. (A) The RT-PCR analyses of the genes upregulated in TPD tree. (B) The RT-PCR analyses of the genes upregulated in healthy tree. (C) The relative expression levels of the genes related to TPD in healthy and TPD trees. The data were collected from the real-time PCR analyses and shown as averages ± SE. The expression level of each gene in healthy tree was defined as 1.0.