Virus and GFP accumulation in 10.6 grafted plants. Extracts from leaves of the scion (S) and rootstock (R) sections of grafted plants consisting of a wild type scion on a 10.6.1 rootstock or of a 10.6.1 scion on a wild type rootstock that was inoculated with wild type PPV after grafting, were subjected to western analysis with specific antibodies (A), as well as to IC-PCR yielding cDNA fragments of 511 nt and 1255 nt for wild type PPV and PPV-NK-GFP, respectively (B). In the case of the plant R1/Swt, extracts from leaves of the rootstock displaying (+ GFP) or not displaying (-GFP) GFP fluorescence were analyzed. Leaves were collected at 39 days after grafting. Samples from a 10.1.7 transgenic plant, expressing the PPV-NK-GFP amplicon from germination, and from wild type N. benthamiana plants healthy (wt Nb) or infected with wild type PPV (wt PPV) were used as controls. Blots stained with Ponceau red showing the Rubisco protein are presented as loading controls in western blot analyses.