Figure 6
From: Constraints to virus infection in Nicotiana benthamiana plants transformed with a potyvirus amplicon
Recovery from virus infection in 10.6.1 transgenic plants. Extracts of mature (M) or young (Y) leaves from 10.6.1 transgenic or wild type N. benthamiana plants inoculated with PPV (panel A), TVMV (panel B) or CMV (panel C) collected at 32 days post inoculation were subjected to western analysis with the indicated antibodies (TVMV antibody also reacts with PPV CP). One mock inoculated 10.6.1 plant that developed an spontaneous infection was also analyzed (panel A). Samples from a 10.1.7 transgenic plant, expressing the PPV-NK-GFP amplicon from germination, and from a healthy N. benthamiana plant (wt Nb) were used as positive and negative controls, respectively. The blots stained with Ponceau red showing the Rubisco protein are presented as loading controls.